ImmuneO2 Protocol




This protocol is supports the immune system and enhances detoxification. It is excellent for:

  • Immune Support
  • Detoxification via perspiration
  • Avascular Tissue Oxygenation
  • Individuals with limited exertion capacity

Hyperthermia from a sauna lifts immunological performance. Oxygen Multistep Therapy documented an increase in oral body temperature to 40°C, increases leukocyte count by 17%, +-7%. See page 224 of Oxygen Multistep Therapy, Sauna Procedure.

The ImmuneO2 Protocol


Goal Immune Modulation — does not overtly up-regulate immunity. von Ardenne reported 17% up-regulation in lymphocytes and measurable increases in motility

Detoxification — Sweat eliminates many toxins.

Avascular Tissue Oxygenation — Elevated temperatures reduce oxygen affinity for hemoglobin. When blood passes through "warmed" areas, oxygen leaves hemoglobin resulting in an increased local concentration of oxygen.
When Used — As follow-up to any other LiveO2 protocol
— In response to immune challenge
— After suspected exposure to infectious agents
— After any airline travel
— At early sign of infectious condition
— After exposure to environmental toxins
— In support of chronic joint discomfort
Difficulty Easy
Detox Intensity Medium — tends to create elevated heart rate for several hours afterwards. Duration of heart rate elevation suggests performance of detoxification.
Time 5 minutes to tolerance
Frequency Daily recommended.
Typical Response — Improved immune function
— Increased body odor indicates detox
— May provoke immune rally at 48 hours
ImmuneO2 Sauna
Required ImmuneO2 Sauna Add to Cart
LiveO2 Adaptive Contrast System
Preferred LiveO2 Adaptive Contrast® System Add to Cart
LiveO2 10L System
Alternate LiveO2 Standard 10L System Add to Cart
LiveO2 10L System
Alternate LiveO2 Standard 5L System Add to Cart

The ImmuneO2 Protocol

The Protocol


  1. Fill the oxygen reservoir to at least 1/3
    • Full reservoir usually not required because of use at rest
    • Respiratory volume usually does not drain reservoir
  2. Put on the mask and connect
  3. Use sauna to tolerance
    • If you have a thermometer, note body temperature
    • Do not exceed 104°F (40°C)
  4. Adaptive Contrast Users
    • Switch to -O2 to increase core blood flow
    • Switch to -O2 to maximize detoxification via perspiration
  5. At exit continue breathing oxygen until pulse drops 100 BPM

Optional Nutrients

  • Thymus Extract 200-500 mg
  • Magnesium Orotate/Aspartate 500 mg
  • Thiamine 100 mg
  • Vitamin C 500 mg
  • 500 mg Arginine Alpha-ketoglutarate

Supplemental nutrients are not usually necessary to achieve the documented protocol effect. Increases in lymphocyte motility are observable without supplementation with Live Blood observation.

The ImmuneO2 Protocol


The immunological aspect of this protocol simulates a fever triggering the physiological adaptations that occur in the body under elevated temperature.

An increase in temperature will normally escalate hemoglobin desaturation causing an increase in oxygen delivery to hyperthermic tissues.

Application of heat lifts immunological performance. Oxygen Multistep Therapy documented an increase in oral body temperature to 104°F or 40°C, increases leukocyte count by 17%, +-7%. See page 224 of Oxygen Multistep Therapy, Sauna Procedure.

Note that a temperature of 104°F, or 40°C, is very high and may be above the tolerance for many indivduals. Modest increases in body temperature are very helpful, and repeated application often result in increases in tolerance.

Availability of at-home hyperthermia devices, IR Saunas, and whole body heating pads enables daily therapeutic use, and lowers stress of hyperthermic immune enhancement. Daily use enables incremental and repeated therapy for cumulative benefit.

Immune Enhancement Protocol

This protocol is extracted from Oxygen Multistep Therapy, except this protocol uses heat to tolerance instead of targeting a fixed core temperature of 104°F or 40°C. This temperature is very high and should be a therapy goal over time.

LiveO2 Hyperthermia Training

Application of heat lifts metabolism. Saunas or hotrooms are well known. A recent method of using a Biomat or whole body heating pad produce similar results by increasing metabolic stress in the presence of oxygen.

Oxygen Multistep Therapy when performed as below increases leukocyte count by 10-24%. This is highly significant in virtually all metabolic syndromes.

Distal Saturation Effect

Hemoglobin Dissociation Curve. Oxygen has lower affinity for hemoglobin as temperature increases.

Oxygen's affinity to hemoglobin decreases as temperature increases. This means that hemoglobin that travels to warmer tissues will be prone to release oxygen as the blood temperature rises to the temperature of the distal tissue.

This decrease will cause hyperthermic tissues to have experience an increase in oxygen delivery due to elevated desaturation. This is the probable reason that "heat" is recommended to accelerate healing in later stages of joint injury recovery.

The ImmuneO2 Protocol


Validation of changes in lymphocyte count are conventionally confirmed with a traditional blood panel. Take a blood test prior to protocol usage. At least one week of daily use of the protocol is suggested. Follow up with another blood test. The difference will likely be relevant.

To reproduce these results with a microscope:

  • Take a blood sample before ImmuneO2 Protocol and video motility of a lymphocyte
  • Do the protocol as directed. Utilize hyperthermia to physical tolerance
  • Take a second blood sample and video the motility of a lymphocyte
  • Compare motility of first and second lymphocytes by video
  • Motility differences normally observable immediately.

The video above shows increased motility of a lymphocyte observed after hyperthermia and oxygen.

The ImmuneO2 Protocol


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