Legacy Protocols

These protocols were extracted and updated from von Ardenne's book, Oxygen Multistep Therapy. They are not medical advice; they are specific exercise recommendations designed to augment oxygen levels targeting specific body systems, like the vascular system and immune system, using different combinations of non-medical approaches.

  • First Time - Before


  • During


  • After


Video Tutor

Advanced Protocols

Before moving on to the advanced protocols, be sure you can comfortably get through the "Quick" protocol as described near the end of the article "The LiveO2 Protocols." The Quick protocol will help you become accustomed to regular exercise, build stamina and restore cellular activity. Once you're able to exercise for a full fifteen minutes with ease, you can choose one or more of the advanced protocols to address specific issues or achieve your particular goals.

The LiveO2 Adaptive Contrast® system provides both oxygen-rich air and oxygen-deprived air. Alternating between these two modes of operation while exercising is the key to driving oxygen deep into the body.


"Sprint" implies high energy output, in essence, to "give it all you've got." This may be done on an exercise bike, tread mill or similar exercise equipment.

The term "target heart rate" means ((220 - your_age) * .7). This is just a guideline; don't worry if you are unable to get your heart rate up to the calculated rate. As with all exercise programs, start slow and work your way up. Over time, your performance will tend to improve.

Preparation Protocol

Use this protocol for at least two weeks before you incorporate oxygen contrast into your regular exercise session. The intent of the protocol is to acclimate the body to physical exertion with lower oxygen pressure. The body adapts to hypobaric exercise by creating more red blood cells, improving lung function and dilating the vascular system.

If you are overly sensitive to breathing "thin" air, you may experience headache, loss of appetite, mild insomnia or other forms of hypoxic discomfort. If you experience any of these or other symptoms, consult your health care provider before continuing this protocol.

  • Set the system to -O2
  • Exercise at a moderate to strong pace for 15 minutes
  • At the end of the 15 minutes, sprint for 30 seconds
  • Switch to +O2 until you've recovered
  • Sprint on +O2 for 30 seconds
  • Recover
  • Sprint on +O2 for 30 seconds
  • Recover and cool down on oxygen for 5 minutes

Hypobaric Recovery

Hypoxic discomfort (altitude sickness) is generally caused by inflammation. The goal of this protocol is to reduce the inflammation.

Recovery Protocol

  • In +O2 mode, bring your heart rate up to 110 beats per minute or higher (about five minutes of exercise is usually sufficient). You will likely feel multiple exertion challenges during this phase
  • Stay on oxygen until you feel comfortable and your oxygen saturation reaches and holds at 99% for 3 minutes or until 15 minutes have elapsed, whichever occurs first
  • If your oxygen stabilizes at 99%, then prepare to do a hypoxic sprint. The goal is to increase your heart rate further and then switch back to oxygen to drive the oxygen deeper into your body
  • Switch to -O2 and sprint for 30 seconds
  • Switch to +O2 until you've recovered
  • Repeat the previous two steps up to three more times or until you have recovered, or until the oxygen reservoir is empty.

Respiratory Inertia Exercise

Hypobaric exercise causes several beneficial effects which occur naturally for people who live at high altitudes:

  • It stimulates the development of lung tissue
  • It stimulates an increase in blood-oxygen transport capacity with a higher number red blood cells
  • The elevated red blood cell counts increase the circulating reserve of oxygen which helps to delay the aerobic to anaerobic energy production during burst exertion
  • Other optimizations occur, but they are not understood at this time.

This protocol transitions the body from an aerobic to an anaerobic state, and back again. Our experience indicates that brief anaerobic sprints stimulate adaptive responses in the body, even though the duration of anaerobic strain is very short, usually less than five minutes in a fifteen minute exercise session.


  • Warm up until you reach your target pulse rate
  • Put on your mask and connect to the oxygen
  • Monitor your pulse and increase intensity to maintain your target pulse rate
  • Hold your target pulse rate for 6 minutes on +O2
  • Switch to -O2 and maintain a constant level of exertion (your pulse and strain will increase)
  • Hold this level until your O2 saturation reaches 90% or drops down to 85% (or until you feel it’s “too much”)
  • Switch back to +O2
  • Hold this high oxygen for 3 minutes
  • Repeat the -O2 interval
  • Finish the session on +O2 until 15 minutes are complete
  • Continue breathing oxygen until pulse drops below 100 bpm.

Saturation Stress Exercise

This protocol optimizes the body’s use of “stored” oxygen by stressing the body’s de-saturation and re-saturation mechanisms.

De-saturation delays the onset of anaerobic energy production by using blood-bound oxygen to prolong aerobic performance.

Re-saturation exercise accelerates recovery from an anaerobic metabolism back to aerobic. For an unknown reason, it seems to accelerate the transition from anaerobic back to aerobic energy.

This protocol adds another control parameter: the target de-saturation level. As you exercise, your body should be able to tolerate lower de-saturation levels during these intervals. As this level goes down, your body is using more of its blood-bound oxygen to delay the transition from aerobic to anaerobic energy production. When you switch to low oxygen, your body will use “stored circulating oxygen” to provide energy.

This protocol establishes a minimum saturation level, defined as the minimum O2 saturation that you can tolerate while under exertion.The lowest level observed in an athlete was 61%, although most individuals capable of sustained physical exertion are capable of 75-85%.

To test your personal de-saturation tolerance:

  • Warm up until you reach your target pulse rate
  • Continue another 5 minutes to make sure the circulatory system is fully adapted to the strain
  • Switch to -O2
  • Monitor your pulse and O2 saturation
  • Sprint at 90% of your maximum ability
  • Monitor your pulse and oxygen saturation levels
  • Continue until you reach exertion tolerance point
  • Record the minimum saturation and highest pulse at the end

Desaturation Exercise Method

  1. Warm up until you reach your target pulse rate
  2. Put on the mask and connect to the oxygen
  3. Exercise for 3 minutes, increasing intensity to maintain your target pulse rate
  4. Switch to -O2
  5. Sprint until you reach respiratory tolerance (note desaturation level and exertion delay)
  6. Switch back to +O2 for 3 minutes (note how long it takes to return to 99%)
  7. Resume at step 5, stop exercising after 15 minutes
  8. Continue breathing oxygen until your pulse drops to 100 bpm or lower.

Clearing Inflammation

Use this protocol after your workout or after a period of hard physical work to clear exertion waste and restore blood flow. This protocol usually cuts recovery time in half. It works by clearing inflammation which retards recovery.

Protocol Goals

  • To restore blood flow to traumatized tissues and enable healing, reversing the inflammation triggered during exercise
  • To reduce systemic waste accumulation to avoid over-exercise fatigue
  • To flush post-performance lactic acid to prevent soreness
  • To maximize body-wide dissolved oxygen to accelerate healing in connective tissue, thus reducing micro-trauma accumulation

Post-Performance Protocol

  1. Warm up until you reach your target pulse rate *
  2. Exercise for 8 minutes at your preferred aerobic output
  3. Do the following steps three times:
    1. Switch to -O2 and do a 30 second sprint
    2. Use -O2 for the first 15 seconds, then switch to +O2 for 15 seconds
    3. Recover on oxygen
    4. If you feel better than when you started, perform these steps again; otherwise, stop now and skip to step 5 below.
  4. Stop exercising at 12 minutes
  5. Continue breathing oxygen until your pulse rate drops to 100 bpm or less.

* If you feel tired, use +O2 setting. If you feel “strong,” then use -O2 to accelerate the warm-up until your heart rate gets to your normal aerobic exercise level.


When the body experiences protracted periods of heavy physical demand, a physiological pattern emerges:

  • Over-exercising creates stress;
  • Stress triggers capillary chokes (See Oxygen Multistep Therapy)
  • Capillary chokes limits blood flow, which in turn increases reliance on anaerobic metabolism
  • Anaerobic metabolism increases the accumulation of lactic acid in muscles and other tissues
  • Excess lactic acid and limited blood flow locks in the anaerobic metabolism
  • This sequence results in ever increasing fatigue and discomfort over time.

The resulting anaerobic state is easily resolved with the following:

  • Exercise until your pulse rate is greater than 120 beats per minute
  • Begin use of oxygen
  • Exercise for 15 minutes
  • Repeat if necessary

This protocol normally resolves fatigue in one session.


The release of lactic acid from tissues may overload the Cori Cycle, resulting in fast bowel for up to three hours after your session.

Brain Oxygenation

This protocol targets restoration of oxygen to the head to help resolve inflammation in the brain, eyes and ears. Many conditions involve inflammation in the brain and sensory organs:

  • Tinnitus – inflammation in the auditory processing of the inner ear
  • Menieres/Vertigo – inflammation in the balance center of the ear
  • Brain Fog – Inflammation in the conscious processing centers of the brain
  • Concussive trauma – inflammation pursuant to head trauma
  • Low Blood Pressure

We have discovered that this protocol substantially and immediately improves mental function using neurological panels.


  • To maximize blood delivery within the brain to reverse inflammation
  • To open all capillaries for optimal blood delivery to brain


  • 500mg Niacin*
  • 500mg Magnesium Orotate
  • 500mg Vitamin C
  • 100mg Thiamine (Vitamin B1)
  • 3000mg Arginine Alpha Ketoglutarate

Brain Oxygenation Protocol

  1. Take supplements and wait 20 minutes
  2. Warm up until you reach your target pulse rate (110 bpm preferred using oxygen)
  3. Exercise for 8 minutes at moderate strain
  4. Do the following steps 3 times:
    1. Switch to -O2 and begin a 30 second sprint
    2. Use -O2 for the first 15 seconds, then switch to +O2 for 15 seconds
    3. Recover on oxygen
    4. Do you feel like your body wants another sprint?, If so, repeat this sequence; otherwise, skip to step 5 below.
    5. Stop exercising at 15 minutes, or when you feel like you need to stop, or when you’ve finished 3 sprints
  5. Continue breathing oxygen until your pulse rate drops to 100 bpm or less.

You can stop at any time. If you are unable to complete the protocol, try again in a few hours or the next day. It may take several passes to become strong enough to complete the protocol. Each attempt will usually make you significantly stronger and more able to continue.

* The purpose of Niacin is to produce a niacin flush. Research this before you start the protocol. You may begin when you are fully comfortable with the protocol and understand what a niacin flush feels like. The niacin flush opens the blood vessels in the skin and head.

Oxygen Detox Protocol

This protocol flushes metabolic waste out of tissues. It also facilitates healing by super-saturating all body tissues, plasma and lymph with very high levels of oxygen. It is equivalent to spending many hours in a hyperbaric chamber. It also boosts the immune system. Use this protocol when you feel sluggish, tired or just want to feel better.

  1. Exercise for 6-8 minutes at a sustainable but aerobic pace (solidly aerobic) (you can use -O2 to accelerate the warm-up)
    • Note exertion challenges – discomfort that occurs at about 1 minute intervals
    • Mentally note the first challenge intensity
    • Reduce effort moderately during challenges
    • Continue on +O2 until challenges become unnoticeable and exertion is easy, usually within 6 to 8 minutes
  2. After exertion challenges end, begin a sprint sequence
    • Switch to -O2
    • Sprint for 15 seconds at your highest level of exertion
    • Switch to +O2 and continue the sprint for another 15 seconds
    • Recover on oxygen until detox clears
  3. Repeat 4 to 8 times
  4. Stop exercising at 15 minutes
  5. Continue breathing oxygen until your pulse drops to 100 bpm or less.

If you start to feel sick, or feel sick with a cold, flu or other immunological challenge (like you spent yesterday on an airplane), then you can augment your immune system by taking:

  • Thymus Extract – 2 capsules
  • Colostrums – 2 capsules
  • Cordyceps Sinensis – 2 capsules

Immune Boost Using a Sauna or Hot Tub

This protocol is for immune challenges that occur from travel-related stress or exposure to cold/flu or other organisms that can compromise performance.

The protocol targets short term saturation of tissues with oxygen, followed by exposure to heat, either in a sauna or hot tub.

Take the following supplements 20 minutes prior to oxygen therapy:

  • Thymus Extract – 2 capsules - helps boost the immune system
  • Colostrums – 2 capsules - supplies immune and growth factors along with a variety of vitamins and minerals
  • Cordyceps Sinensis – 2 capsules - helps the body resist fatigue and boosts the immune system

Immune Boost Protocol Steps

  • Take supplements and wait 20 to 40 minutes
  • Warm up until you reach your target pulse rate
  • Put on the mask and connect to the oxygen
  • Exercise for 10 minutes with moderate effort until you feel hot and start to sweat
  • Move into the sauna or hot tub, continuing oxygen use, if possible
  • Remain in the sauna or hot tub until your body reaches 104 degrees or tolerance (do not exceed 104)
  • Exit the sauna or hot tub and cool down, continue to breathe oxygen until your pulse drops below 90 bpm.

Connective Tissue

This protocol accelerates connective tissue development in situations where prior exercise has caused a connective tissue strength imbalance such that the muscle is stronger than the tissue which connects it to the bone.


Many exercise methods that focus on muscle development often create a strength imbalance between muscle and connective tissue. In simple terms, the connective tissue must be able to withstand the maximum repetitive jerk-tension of the muscle without cumulative micro-trauma.

Modern exercise methods do not recognize or address the factors that enable connective tissue development. This method is based primarily on the observation that white tissue does not have capillaries.


There are two unrecognized effects that determine the rate of connective tissue development versus muscle development:

  • Connective tissue receives oxygen and nutrients from extra-cellular fluids, while muscle obtains nutrients and oxygen from blood;
  • Connective tissue growth is stimulated by shock, while muscle development is stimulated by smooth strain.

As a result, exercise methods that do not balance these four factors often produce structure and strength imbalances that result in a vulnerability to injury.

These effects particularly affect large athletes with well developed muscle. They often exhibit a higher joint-injury vulnerability because strong muscles over-stress the connective tissue, resulting in chronic inflammation and elevated connective tissue injury.

Strength exercise methods, weight lifting in particular, increases muscle mass much more rapidly than connective tissue strength.

This method enables specialized development of connective tissue strength and durability.

Principles of Method

There are two simple principles of this exercise method:

  • Saturate non-vascular body fluids with oxygen
  • Exercise using methods that amplify shock, but not smooth exertion.

Physically, this suggests the use of high oxygen while lifting moderate weight, in motion patterns that isolate vulnerable connective tissue from muscles and ligaments.

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